The solution is most active for one hour after mixing.  Be sure to apply the solution within this time period for maximum effectiveness.
	Do not enclose the mixture in an airtight container, or you could have a big mess on your hands!  The reason for this is because when the ingredients are combined they release oxygen (Remember making "volcanoes" out of baking soda and vinegar when you were a little kid?  Similar principle here.).
	Two alternate methods of applying the solution are using a spray bottle or a garden watering can.  A spray bottle works best for applying the solution to hard surfaces like concrete or linoleum, or to vertical surfaces like walls or shower tile (This is not the best method for carpet.).  Just be sure to thoroughly spray the area you are treating.
	A garden watering can is best for medium to large areas--indoors or outdoors.
	For really tough odors, you may have to treat the area more than once.  Carpet, furniture and mattresses typically require 1-2 treatments.  Concrete, wood, tile and other hard surfaces usually need 3-5 treatments (1 treatment per day).
	For concrete and other hard surfaces, you want to make enough of the recipe to completely cover the stain.  Pour or spray the solution on and let it soak, soak, soak.  Do not mop or wipe up.  Let it sit for 24 hours (depending on the humidity level, it will usually evaporate during this time).  Repeat the treatment once a day for 3-5 days.
	For mattresses, the best thing to do is to make a double, triple or even quadruple batch of the recipe--enough to completely cover the stain--and then really pour it on. Don't be afraid of saturating the mattress. The urine has gone deep into the mattress, so you need to make sure that there's enough solution to go deep into the mattress as well. The solution has to reach the urine to neutralize it.  We recommend treating the mattress in the morning so that the solution can work through the day.  The mattress will often be dry enough for use by bedtime.